Let's talk Tylenol Paracetamol (acetaminophen) There are many side effects that most people don't realize. OTC does not mean it is side effect free, especially with chronic use. Even just 4 days of Sex differences in glutathione metabolism and acetaminophen toxicity Acetaminophen induces liver injury and depletes glutathione in mice brain: Prevention by Moringa oleifera extract ScienceDirect Tylenol's Impact on Glutathione and Liver Damage View of Acetaminophen, the missing anion gap in the patient with metabolic acidosis The Southwest journal of medicine Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.